Bovine Serum Albumin (BSA) is commonly used in cell culture protocols, particularly where protein supplementation is necessary and the other serum components are unwanted.
In cell culture, it acts as a small molecule carrier.
Because of its negative charge, Bovine Serum Albumin:
- Binds water, salts, fatty acids, vitamins and hormones and carries these bound components between tissues and cells.
- Its binding capacity also makes Bovine Serum Albumin an effective scavenger removing toxic substances, including pyrogens, from the medium.
- It is readily soluble in water and can only be precipitated by high concentrations of neutral salts such as ammonium sulfate.
The solution stability of Bovine Serum Albumin is excellent especially if the solutions are stored as frozen aliquots.
- Albumin is used to solubilize lipids, and it is also used as a blocking agent in Western Blot or ELISA applications.
- Albumins are frequently used as stabilizers for other solubilized proteins (e.g., labile enzymes). However, albumin is readily coagulated by heat.
When heated to 50°C or above, albumin quite rapidly forms hydrophobic aggregates which do not revert to monomers upon cooling.
Aggregation is also expected to occur at somewhat lower temperatures but at a relatively slower rate.
- Discover the range of Biowest Bovine Serum Albumin: