DMEM – F12 w/o L-Glutamine w/ 25 mM Hepes – L0096

HEPES Sodium Bicarbonate NEAA Calcium Magnesium Phenol red Sodium Pyruvate

DMEM – F12 w/o L-Glutamine w/ 25 mM Hepes – L0096

Developed initially to study hormonal requirements of cells in culture, DMEM-Ham’s F12 is used as basal medium for growing many different mammalian cells (MDCK, fibroblasts, endothelial cells). DMEM – Ham’s F12 is a mixture (1:1) of DMEM and Ham’s F12 medium.

Its formulation is L-Glutamine-free and contains Hepes (25mM).

Other packagings available on request

For your special formulation, please contact us, we can make your custom-made media on request

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Cat number
L0096
Description
reduce
develop
Product code L0096
Shipping conditions Room temperature or refrigerated transport
State Liquid
Shelf life 12 months
Storage +2/+8°C.
Sterility Sterile filtered
Applications
reduce
develop

Cell culture

Formulation is without L-Glutamine, with Hepes (25mM), sodium pyruvate and phenol red
Glucose concentration : 3.151g/L

To supplement with L-Glutamine,
Addition of L-Glutamine 100X, 200mM solution (ref: X0550) : 12.5 ml/l
Addition of L-Glutamine powder (ref: P1031) : 0.365 g/l

Supplements should be added to the medium as sterile supplements.

Our Biowest Cell Culture Media

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In the service of science since 1987

Founded in 1987, Biowest is the European leader in the collection of animal sera, providing the widest range of sera and cell culture media available on the market.

Our products are employed in the production of biopharmaceuticals and vaccines and in the academic, governmental and industrial research sectors in fields spanning the areas of cell biology, genomics, proteomics, virology, immunology, innovative drug research and toxicology.

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In the service of science since 1987