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In 1976, Guilbert and Iscove demonstrated that precursor cells of erythrocytes and macrophages could be cultured in a reduced-serum medium supplemented with albumin, transferrin, lecithin, and selenium.
Iscove's Medium is a variation of Dulbecco's Modified Eagle's Medium (DMEM) containing:

  • Selenium, additional amino acids and vitamins
  • Sodium pyruvate
  • HEPES buffer
  • Potassium nitrate instead of ferric nitrate.

Further studies demonstrated that Iscove's Medium would support:

  • Murine B lymphocytes
  • Hematopoietic tissue from bone marrow
  • B cells stimulated with lipopolysaccharide
  • T lymphocytes
  • a variety of hybrid cells.
     
  • Discover the range of Biowest Iscove's Media: