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Leibovitz L-15 Medium formulation promotes cell growth in CO2 unbalanced medium.
The formulations were developed with the sodium bicarbonate buffer.
The Leibovitz L-15 Medium is buffered with a complement of salts, free base amino acids and galactose, so it can be used under conditions of free gaseous exchange with the atmosphere.

When properly supplemented, Leibovitz L-15 Medium supports existing cell lines, such as HEp-2 and LLC-MK2, as well as primary embryonic and adult human explanted tissue.
Many viruses have also been successfully cultivated in this medium.