Mycokit is designed to detect the presence of mycoplasma contaminating biological materials, such as cultured cells.
Mycoplasma detection by the usual culture procedure is time consuming.
Some mycoplasma species are difficult to cultivate.
With PCR testing, results are obtained within only a few hours, since the presence of contaminant mycoplasma can be detected simply by verifying the bands of amplified DNA fragments in electrophoresis.
There is no need to:
- Prepare probes labelled with radioisotopes
- Calculate enzyme, dNTPs or buffer concentrations
Instead, a ready-to-use optimized PCR mix is provided.
The reaction mix is supplemented with a precipitant that allows direct loading of PCR products into agarose gel.
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