EPC

epc cells

The endothelial progenitor cells (EPCs) are immature cells, present in peripheral blood, where their physiological role is the maintenance of vascular integrity. They are derived from bone marrow, then enter in the blood, where they constitute a rare population of cells. They are integrated in the formation of new vessels, for example during the process of revascularization following a vascular lesion. In culture, these cells form colonies of endothelial cells, which quickly acquiring a strong power of proliferation.

EPCs can be isolated from umbilical cord blood, where their number is about 10 times higher than that of adult peripheral blood, and they have a greater proliferation than that isolated drom adult peripheral blood.

These endothelial cells derived from EPC have several advantages:

  • Their capacity to proliferate is higher than that of HUVEC (Human Umbilical Vein Endothelial Cells): 45 population doublings for EPCs versus 22 population doublings for HUVEC (figure 1).
  • They are stable in culture: They can be cultured for more than 80 days without changing their phenotype or showing signs of senescence or activation markers (ICAM-1, VCAM-1 nor P-Selectin)
  • They have not undergone a process of specialization, for example arterial or venous, because they are derived from immature progenitors
  • They have all the functions of endothelial cells.
    • They are activated by TNF-α, induced to overexpress the adhesion molecules ICAM-1 and VCAM-1
    • They incorporate acetylated LDL
    • They form a network of vascular tubes on Matrigel ®.
    • They are able to migrate under the action of a chemoattractant (VEGF)


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Frozen EPC Cord blood - Biowest, The Serum Specialist

Frozen EPC Cord blood

Product Code : C4503
State : Frozen
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